產(chǎn)品名稱：anti-Flag Tag Mouse Monoclonal antibody（鼠單克隆抗體）
產(chǎn)品型號(hào)：M1001060
產(chǎn)品報(bào)價(jià)：
產(chǎn)品特點(diǎn)：anti-Flag Tag Mouse Monoclonal antibody（鼠單克隆抗體）在Western Blotting中使用內(nèi)參其實(shí)就是在WB過程中的另外用內(nèi)參對(duì)應(yīng)的抗體檢測(cè)內(nèi)參，這樣在檢測(cè)目的產(chǎn)物的同時(shí)可以檢測(cè)內(nèi)參的表達(dá)，由于內(nèi)參在各組織和細(xì)胞中的表達(dá)相對(duì)恒定，借助檢測(cè)每個(gè)樣品內(nèi)參的量就可以用于校正上樣誤差，這樣半定量的結(jié)果才更為可信。
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英文名稱 anti-Flag Tag Mouse Monoclonal antibody
應(yīng)用 WB 1:2500 IP 1:100 IF:1:500 Optimal dilutions should be determined by the end user.
規(guī) 格 100ul
濃 度 1mg/ml
保 存 Store at -20°C. Do not aliquot the antibody.
儲(chǔ)存液 PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol
純化方法 affinity purified by Protein A
亞 型 IgG
性 狀 Liquid
克隆類型 monoclonal
宿 主 Mouse
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
說明書
Anti- Flag Tag Mouse Monoclonal Antibody (1B3)
Catalog No. M1001060
Size：100μl
Storage: Store at -20°C. Do not aliquot the antibody.
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to
interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively
as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to
separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can
also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many
different assays that require recognition by an antibody. If there is no antibody against the studied protein,
adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag
sequence.
Molecular Weight: N/A
Application: WB 1:2500 IP 1:100 IF:1:500 Optimal dilutions should be determined by the end user.
Specificity: The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag
fusion proteins.
Form: Liquid ,1mg/ml
Storage buffer: PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol
Relative Products
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M1001030 Anti-GFP Tag Mouse Monoclonal Antibody(2H7)
M1001040 Anti-GST Tag Mouse Monoclonal Antibody(2C7)
M1001050 Anti- cMyc Tag Mouse Monoclonal Antibody(2C5)
M1001060 Anti- Flag Tag Mouse Monoclonal Antibody(1B3)
RG001010 Anti- HA Tag Rabbit Polyclonal Antibody
RG001020 Anti- His Tag Rabbit Polyclonal Antibody
RG001030 Anti- GFP Tag Rabbit Polyclonal Antibody
RG001040 Anti- GST Tag Rabbit Polyclonal Antibody
RG001050 Anti- cMyc Tag Rabbit Polyclonal Antibody
RG001060 Anti- Flag Tag Rabbit Polyclonal Antibody
Solarbio
Beijing Solarbio Science & Technology Co., Ltd
/82
Http://www.solarbio。。ｃｏｍ
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