產(chǎn)品名稱(chēng):碘化丙啶PI 細(xì)胞檢測(cè)
產(chǎn)品型號(hào):P8080
產(chǎn)品特點(diǎn):碘化丙啶PI 細(xì)胞檢測(cè)貨號(hào):P8080規(guī)格:10mg/支CAS:25535-16-4分子式:C 27 H 34 I 2 N 4分子量:668.39儲(chǔ)存條件:2-8℃保存純度:≥95%外觀(性狀):紅色粉末單位:支
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碘化丙啶PI 細(xì)胞檢測(cè)
貨號(hào):P8080
規(guī)格:10mg/支
碘化丙啶PI 細(xì)胞檢測(cè)
說(shuō)明:熒光染料PI(碘化丙啶)是一種可對(duì)DNA染色的細(xì)胞核染色試劑,常用于細(xì)胞凋亡檢測(cè),英文全稱(chēng)是Propidium Iodide。它是一種溴化乙啶的類(lèi)似物,在嵌入雙鏈DNA后釋放紅色熒光。盡管PI不能通過(guò)活細(xì)胞膜,但卻能穿過(guò)破損的細(xì)胞膜而對(duì)核染色。PI經(jīng)常被用來(lái)與Calcein-AM或者FDA等熒光探針一起使用,能同時(shí)對(duì)活細(xì)胞和死細(xì)胞染色。PI-DNA復(fù)合物的激發(fā)和發(fā)射波長(zhǎng)分別為535 nm和615 nm。
產(chǎn)品說(shuō)明:
碘化丙啶(PI)是一種核酸熒光染料,它不能透過(guò)完整的細(xì)胞膜,但能夠透過(guò)死細(xì)胞和凋亡中晚期細(xì)胞的細(xì)胞膜而使細(xì)胞核染色。因此,PI 作為熒光探針常用于細(xì)胞凋亡(apoptosis)或細(xì)胞壞死(necrosis) 的檢測(cè)。在流式細(xì)胞分析中,PI 常與其他染料如 Calcein-AM、Hoechst 33258或 Hoechst 33342 聯(lián)合使用,來(lái)區(qū)分凋亡早晚期細(xì)胞以及死細(xì)胞。此外,PI 也常作多色熒光染色的復(fù)染劑使用。
產(chǎn)品用途:
用于細(xì)胞凋亡(apoptosis)或細(xì)胞壞死(necrosis)的檢測(cè),常用于流式細(xì)胞儀分析;常被用來(lái)與 Calcein, AM、Hoechst 33258 或 Hoechst 33342 等一起使用,能同時(shí)對(duì)活細(xì)胞和死細(xì)胞染色。
使用方法: 可溶于水或 DMSO。染色過(guò)程如下:
1)用 PBS 或適當(dāng)?shù)木彌_液制備 10~50μM 的 PI 溶液。
2)將 1/10 培養(yǎng)基體積的 PI 溶液加入到細(xì)胞培養(yǎng)基中(也可以用 1/10 濃度的 PI 緩沖液代替培養(yǎng)基)。
3)在 37℃培養(yǎng)細(xì)胞 10~20 分鐘。
4)用 PBS 或合適的緩沖液洗滌細(xì)胞兩次。
5)用 535nm 激發(fā)波長(zhǎng),615nm 發(fā)射波長(zhǎng)的濾光器的熒光顯微鏡觀察細(xì)胞。
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